Identification of bacteriophage types and their carriage in <I>Staphylococcus aureus</I>
| Authors | |
|---|---|
| Year of publication | 2004 |
| Type | Article in Periodical |
| Magazine / Source | Archives of Virology |
| MU Faculty or unit | |
| Citation | |
| Web | |
| Field | Genetics and molecular biology |
| Keywords | Staphylococcus aureus bacteriophages; lysogeny; prophage detection; multiplex PCR; bacteriophage genomics |
| Description | Conserved genomic sequences distinctive of Staphylococcus aureus phage species 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages to species, but also to detect prophages in S. aureus genomes. One to four different prophages were unambiguously detected in experimentally lysogenized S. aureus strains, and substantial variation in prophage content was found in 176 S. aureus clinical strains of different provenance. In addition, by using a comparative genomics approach, all the prophages in the S. aureus genomes sequenced to date could be revealed and classified to phage species |
| Related projects: |