Identification of bacteriophage types and their carriage in <I>Staphylococcus aureus</I>

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Authors

PANTŮČEK Roman DOŠKAŘ Jiří RŮŽIČKOVÁ Vladislava KAŠPÁREK Petr ORÁČOVÁ Eva KVARDOVÁ Veronika ROSYPAL Stanislav

Year of publication 2004
Type Article in Periodical
Magazine / Source Archives of Virology
MU Faculty or unit

Faculty of Science

Citation
Web
Field Genetics and molecular biology
Keywords Staphylococcus aureus bacteriophages; lysogeny; prophage detection; multiplex PCR; bacteriophage genomics
Description Conserved genomic sequences distinctive of Staphylococcus aureus phage species 3A, 11, 77, 187 and Twort, representative of phage serogroups A, B, F, L and D, were identified and characterized. PCR primers designed for the above sequences were used for development of a multiplex PCR assay which enabled us not only to classify all phages of the International Typing Set plus 16 additional phages to species, but also to detect prophages in S. aureus genomes. One to four different prophages were unambiguously detected in experimentally lysogenized S. aureus strains, and substantial variation in prophage content was found in 176 S. aureus clinical strains of different provenance. In addition, by using a comparative genomics approach, all the prophages in the S. aureus genomes sequenced to date could be revealed and classified to phage species
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