Solid-phase reversed immobilization of bacterial DNA from aqueous two phase system

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Authors

RITTICH Bohuslav ŠPANOVÁ Alena NĚMCOVÁ Petra HORÁK Daniel

Year of publication 2007
Type Article in Proceedings
Conference Ninth International Symposium on Advances in Extraction Technologies
MU Faculty or unit

Faculty of Science

Citation
Field Genetics and molecular biology
Keywords DNA; isolation; magnetic microspheres; P(HEMA-co-EDMA); aqueous two-phase system; PEG 6000; NaCl
Description The aim of work was the isolation of PCR-ready DNA from dairy products by magnetic hydrophilic poly(2-hydroxyethyl methacrylate-co-ethylene dimethylmethacrylate) (P(HEMA-co-EDMA)) microspheres in the presence of an aqueous two-phase system (composed of PEG 6000 and NaCl). DNA was apparently adsorbed on magnetic particles surface from two-phase aqueous (PEG 6000/NaCl) system. The sensitivity of PCR of the DNA isolation method tested was comparable with the phenol extraction method.
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