Seasonal changes in complement activity of common carp (Cyprinus carpio)
| Authors | |
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| Year of publication | 2008 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | The fish non-specific immune response includes cellular (phagocytes) and humoralparts (complement system mainly) or the systemic inflammation. Complement system is composed of more than 30 proteins and three pathways of its activation have been recognised: classical, alternative and possible lectine. The total bacteriolytic activity (TA) including all pathways and activity of the alternative pathway (AP) were assessed in the plasma of common carp, Cyprinus carpio (Cyprinidae). The samples were collected in five periods of one year including different seasons and all together 80 males and 80 females were used. Bioluminescence-based method of complement activity uses bioluminescent recombinant strain of E. coli; there is a correlation between light production and viability. The time needed for 50% killing of bacteria by plasma sample was used as the measured parameter. Plasma in concentration 300microlitrel/ml needed approx. 1.5 hours in TA; AP showed lower bacteriolytic activity (approx. 1.7 hours) and moreover, needed higher plasma concentration (500microlitrel/ml). The seasonal changes in complement activity were observed, the highest TA was determined in autumn, lower in the summer. This activity in spring and winter was the lowest, nevertheless very similar each other. Concerning AP the highest activity was detected in autumn, lower in spring and the lowest values were determined in winter and in summer. |
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