Prediction of localization and interactions of apoptotic proteins

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Authors

VAŘECHA Miroslav ZIMMERMANN Michal AMRICHOVÁ Jana ULMAN Vladimír MATULA Pavel KOZUBEK Michal

Year of publication 2009
Type Article in Periodical
Magazine / Source Journal of Biomedical Science
MU Faculty or unit

Faculty of Informatics

Citation
Field Genetics and molecular biology
Keywords AIF;apoptosis-inducing factor;AMID;AIF-homologous mitochondrion-associated inducer of death;endonuclease G;heat shock protein;DNA topoisomerase II;CPS-6;CED-3 protease suppressor;WAH-1
Description We found the AMID protein to be cytoplasmic, most probably incorporated into the cytoplasmic side of the membranes of various organelles. The highest concentration of AMID was observed associated with the Golgi. We did not observe its translocation into the nucleus during apoptosis. The proposed role of AMID in apoptosis was thus not observed. Bioinformatic predictions were conducted to analyze the interactions of the studied proteins with each other and with other possible partners. We conducted molecular modelling of proteins with unknown 3D structure: endonuclease G, CPS-6, WAH-1, and HSP70-1. These models were then refined by MolProbity server and employed in molecular docking simulations of interactions. Our results present new data about the structure, localization, functions and interactions of proteins AMID, AIF, endonuclease G, and other proteins.
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