Potenciometrická studie fyziologicky významných derivátů adeninu
| Title in English | Potentiometric study of physiologically important derivatives of adenine |
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| Authors | |
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | XXX. moderní elektrochemické metody - sborník přednášek |
| MU Faculty or unit | |
| Citation | |
| Field | Analytic chemistry |
| Keywords | purine derivatives; potentiometry; protonation constant |
| Description | There are currently intensively studied adenine derivatives known as cytokinins, which are plant hormones which promote plant growth, bud formation and branching stems. Important derivatives of cytokinins olomoucine, roscovitine, bohemine act as inhibitors of cyclin dependent kinases, thereby preventing cell division, and from this perspective, it appears as a potential cytostatics. The aim of this work was the thermodynamic study of protonisation of adenine, 6 benzylaminopurine and its methoxy and chlorine derivatives. Acid/base potentiometric titration allows to determine the equilibrium constants of protonisation pKa and determine the impact of substituent and its position on the benzyl. pKa dependence on temperature (15 to 37 C) and ionic strength (I = 0.1 M) are the basis for the thermodynamic evaluation of changes in enthalpy and entropy of the protonisation process. All the potentiometric determination of purine derivatives for reasons of solubility conducted in an environment of 10% (v/v) CH3OH in water. |
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