Determination of novel tumor markers in prostate carcinoma

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Authors	MASAŘÍK Michal GUMULEC Jaromír KUCHTÍČKOVÁ Šárka KUDLÁČKOVÁ Veronika JURAJDA Michal PAVLÍK Dušan ROVNÝ Arne HRABEC Roman KŘÍŽKOVÁ Soňa KIZEK René
Year of publication	2010
Type	Conference abstract
MU Faculty or unit	Faculty of Medicine
Citation
Description	Prostate cancer is second leading cause of death among men. Metallothionein (MT) and alpha-methylacyl-CoA (AMACR) racemase could be such markers. Expression of bothof these proteins is highly unregulated in prostate cancer. MT levels are elevated most likely due to disbalance of zinc metabolism in this disease, and reason of elevation of AMACR is still unknown. In our work we analyzed levels of MT, PSA and AMACR in cell lines derived from prostate carcinoma - LNCaP, PC-3 and 22RVL, which were compared to prostate cell line PNT1A derived from normal prostate epithelium. For analysis we used immunoseparation techniques, western blotting and SDS-PAGE. In addition to this clasical methods of molecular biology we used novel electrochemical detection and capillary electrophoresis on the chip.
Related projects:	Analýza metalothioneinu u karcinomu prostaty na úrovni DNA, RNA a proteinu Nádorové markery, jejich stanovení a korelace s karcinomem prostaty