ELECTROCHEMICAL STUDY OF SHORT CYTOSINE OLIGONUCLEOTIDES

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Authors	GURECKÝ Libor BALCAROVÁ Zdeňka PILAŘOVÁ Iveta TRNKOVÁ Libuše
Year of publication	2012
Type	Article in Proceedings
Conference	XII. Pracovní setkání fyzikálních chemiků a elektrochemiků
MU Faculty or unit	Central European Institute of Technology
Citation
Field	Physical chemistry and theoretical chemistry
Keywords	cytosine;oligonucleotides;cyclic voltammetry;elimination voltammetry
Description	Cytosine-rich oligodeoxynucleotides (C-rich ODNs) play diverse roles in the regulation of protein expression at both transcriptional and translational levels. A repetitive C sequence can be responsible for some diseases. It is commonly known that C-rich ODNs form the stable i-motif structure, interesting due to a crucial role in the process of genetic information transfer. The aim of this contribution is to find out the relationship between the structure of homo-ODNs in buffered solutions with the same ionic strength (I = 0.18 M) but with a different number of cytosines (dCx; x = 3, 4, 5, 6, 9). Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS), electrochemical reduction signals studied on a mercury electrode reflect the conformation changes of the C-rich ODN chain.
Related projects:	Víceúrovňový design pokrokových materiálů CEITEC - Central European Institute of Technology