Project information
Czech Infrastructure for Integrative Structural Biology
(CIISB)
- Project Identification
- LM2015043
- Project Period
- 1/2016 - 12/2019
- Investor / Pogramme / Project type
-
Ministry of Education, Youth and Sports of the CR
- Large Infrastructures for Research, Development and Innovation
- MU Faculty or unit
-
Central European Institute of Technology
- prof. RNDr. Vladimír Sklenář, DrSc.
The Czech Infrastructure for Integrative Structural Biology (CIISB) is a distributed infrastructure of the core facilities and central laboratories of CEITEC (Central European Institute of Technology, Brno) and BIOCEV (Biotechnology and Biomedicine Centre, Vestec, Prague-West) providing expertise and access to technologies used for integrative approaches to structural analysis of biologically important cellular components and macromolecules - proteins, nucleic acids, and their complexes. CIISB, equipped with the cutting-edge technologies (total investments will exceed 750 mil. CZK by the end of 2015), will offer access to the equipment and expertise in the following fields: high-field NMR spectroscopy; high throughput crystallization of biological macromolecules; X-ray diffraction techniques; Bio-SAXS measurements; AFM imaging; characterization of proteins, nucleic acids and complexes by biophysical methods including microcalorimetry, dynamic light scattering, and surface plasmon resonance; high-end cryo-electron microscopy and tomography allowing studies of cellular structures, organelles, and biomacromolecular complexes by 3D imaging; protein expression in eukaryotic cells; high-end mass spectrometry characterization of biomacromolecules; determination of protein post-translational modifications; MS-based peptide mapping/sequencing; high throughput sequencing and proteomic services. CIISB infrastructure aims to provide a high quality open access services in provision of methods and techniques on a level of national and trans-national importance. CIISB as National Affiliated Center is a part of the ESFRI projects INSTRUCT.
Publications
Total number of publications: 313
2019
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Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis
Proteomics, year: 2019, volume: 19, edition: 21-22, DOI
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Quantitative Biology of Human Shelterin and Telomerase: Searching for the Weakest Point
International Journal of Molecular Sciences, year: 2019, volume: 20, edition: 13, DOI
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Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase
Journal of the American Chemical Society, year: 2019, volume: 141, edition: 42, DOI
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Quantum chemical calculations of P-31 NMR chemical shifts of P-donor ligands in platinum(II) complexes
Journal of Molecular Modeling, year: 2019, volume: 25, edition: 11, DOI
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Receptor-Independent Transfer of Low Density Lipoprotein Cargo to Biomembranes
NANO LETTERS, year: 2019, volume: 19, edition: 4, DOI
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Selectively Oxidized Cellulose with Adjustable Molecular Weight for Controlled Release of Platinum Anticancer Drugs
Biomacromolecules, year: 2019, volume: 20, edition: 4, DOI
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SERPING1 exon 3 splicing variants using alternative acceptor splice sites
Molecular Immunology, year: 2019, volume: 107, edition: MAR 2019, DOI
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Simultaneous study of mechanobiology and calcium dynamics on hESC-derived cardiomyocytes clusters
Journal of Molecular Recognition, year: 2019, volume: 32, edition: 2, DOI
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Solvothermal hot injection synthesis of core-shell AgNi nanoparticles
Journal of Alloys and Compounds, year: 2019, volume: 770, edition: JAN, DOI
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Staphylococcus petrasii diagnostics and its pathogenic potential enhanced by mobile genetic elements
International Journal of Medical Microbiology, year: 2019, volume: 309, edition: 8, DOI