Validation of capillary electrophoretic method for in-capillary metabolism of dextromethorphan
| Authors | |
|---|---|
| Year of publication | 2010 |
| Type | Article in Proceedings |
| Conference | Chiranal 2010 |
| MU Faculty or unit | |
| Citation | |
| Field | Biochemistry |
| Keywords | dextromethorphan - cytochrome P450 2D6 - in-capillary rection - CE |
| Description | Metabolism pathway of dextromethorphan (DEX) was chosen as the example of drug transformation under the action of cytochrome P450 2D6 (CYP 2D6). CYP 2D6 belongs to the important enzymes of all CYP isoforms as it is involved in metabolism of about 25% of all commonly prescribed drugs. Recombinant human CYP enzymes have an important role in elucidating the biotransformation of new drug candidates. Capillary electrophoresis (CE) has become a common analytical tool in drug analysis for qualitative and quantitative determinations. Moreover, CE enables to conduct in-capillary homogenous enzymatic reactions. We intended to develop in-capillary CYP 2D6 enzymatic assay. The validated range is acceptable for examination of parameters of stated enzymatic reaction. |
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