Structural analysis of delta-subnit of RNA-polymerase by X-ray

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Authors	DEMO Gabriel PAPOUŠKOVÁ Veronika SANDEROVA Hana WIMMEROVÁ Michaela
Year of publication	2011
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	RNA polymerase (RNAP) is an essential enzyme that is responsible for transcription of DNA into RNA. The recombinant form of delta-subunit from Bacillus subtilis is 173 aa long protein. The structure of N-terminal domain was determined by NMR. Crystallization and structure determination by X-ray analysis were also chosen. Crystals were obtained after 7 to 9 days with an average size 0.5 mm x 0.4 mm. Diffraction data were collected at ESRF Grenoble, ID 23-1.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Molekulární a strukturní biologie vybraných cytostatik. Od mechanických studií k chemoterapii rakoviny Delta podjednotka RNA polymerázy z Gram pozitivních bakterií