Project information
Vnitřní vlastnosti RAD51 vlákna a jeho biologické regulace

Information

This project doesn't include Faculty of Education. It includes Faculty of Medicine. Official project website can be found on muni.cz.

Project Identification

GA17-17720S

Project Period

1/2017 - 12/2019

Investor / Pogramme / Project type

Czech Science Foundation

Standard Projects

MU Faculty or unit

Faculty of Medicine

doc. Mgr. Lumír Krejčí, Ph.D.
Mgr. Veronika Altmannová, Ph.D.
Bc. Ondrej Beláň
Mgr. Lenka Hejmalová
RNDr. Mário Špírek, PhD.

Homologous recombination is an essential mechanism for the repair of DNA double-strand breaks and damaged replication forks. HR is initiated by the formation of a RAD51 helical nucleoprotein filament on single stranded DNA, which is able to search for and invade homologous duplex DNA to form a joint molecule. Although, RAD51 is unable to work alone and is reliant on co-factors (BRCA2 and RAD51 paralogs,) to drive the HR reaction to completion a mechanistic understanding of how this is achieved is unclear. This proposal will bring together the unique expertise to investigate how the properties of the RAD51 nucleoprotein filament are modulated by HR co-factors. Through the integration of real-time kinetic measurements, microscale thermophoresis, single molecule and structural visualization studies, conformational dynamics, and validation in biological systems we will provide unprecedented insight into the mechanisms by which HR co-factors impact on the HR reaction and its relevance to development of cancer.

Publications

Total number of publications: 14

2018

SAMHD1 acts at stalled replication forks to prevent interferon induction

COQUEL Flavie SILVA Maria Joao TECHER Hervé ZADOROZHNY Karina SHARMA Sushma NIEMINUSZCZY Jadwiga METTLING Clément DARDILLAC Elodie BARTHE Antoine SCHMITZ Anne Lyne PROMONET Alexy CRIBIER Alexandra SARRAZIN Amélie NIEDZWIEDZ Wojciech LOPEZ Bernard COSTANZO Vincenzo KREJČÍ Lumír CHABES Andrei BENKIRANE Monsef YEA-LIH Lin PASERO Philippe

Article in Periodical

Nature, year: 2018, volume: 557, edition: 7703, DOI

2017

Fanconi-Anemia-Associated Mutations Destabilize RAD51 Filaments and Impair Replication Fork Protection

MOVSESJAN Karina SANNINO V. BELÁŇ Ondrej MLČOUŠKOVÁ Jarmila ŠPÍREK Mário COSTANZO V. KREJČÍ Lumír

Article in Periodical

Cell Reports, year: 2017, volume: 21, edition: 2, DOI
RECQ5 Helicase Cooperates with MUS81 Endonuclease in Processing Stalled Replication Forks at Common Fragile Sites during Mitosis

DI MARCO Stefano HAŠANOVÁ Zdenka KANAGARAJ Radhakrishnan CHAPPIDI Nagaraja ALTMANNOVÁ Veronika MENON Shruti SEDLÁČKOVÁ Hana LANGHOFF Jana SURENDRANATH Kalpana HÜHN Daniela BHOWMICK Rahul MARINI PALOMEQUE María Victoria FERRARI Stefano HICKSON Ian D. KREJČÍ Lumír JANSCAK Pavel

Article in Periodical

Molecular Cell, year: 2017, volume: 66, edition: 5, DOI
Smarcal1-Mediated Fork Reversal Triggers Mre11-Dependent Degradation of Nascent DNA in the Absence of Brca2 and Stable Rad51 Nucleofilaments

KOLINJIVADI A.M. SANNINO V. DE ANTONI A. MOVSESJAN Karina KILKENNY M. TECHER H. BALDI G. SHEN R. CICCIA A. PELLEGRINI L. KREJČÍ Lumír COSTANZO V.

Article in Periodical

Molecular Cell, year: 2017, volume: 67, edition: 5, DOI