Project information
Single cell analysis: a modern tool to study clonal evolution in high-risk patients with chronic lymphocytic leukemia (Single cell analysis of CLL cells)

Information

This project doesn't include Faculty of Education. It includes Central European Institute of Technology. Official project website can be found on muni.cz.

Project Identification

NU20-08-00314

Project Period

5/2020 - 12/2023

Investor / Pogramme / Project type

Ministry of Health of the CR

Ministry of Health Research Programme 2020 - 2026
Subprogram 1 - standard

MU Faculty or unit

Central European Institute of Technology

prof. RNDr. Šárka Pospíšilová, Ph.D.

Cooperating Organization

University Hospital Brno-Bohunice

Responsible person Mgr. Jana Kotašková, Ph.D.

The proposed project builds on continuous well established research of our team in the field of hematologic malignancies. In this study we would like to profound our knowledge and understanding the mechanisms underlying clonal evolution of chronic lymphocytic leukaemia (CLL) cells. We have already studied CLL using variety of bulk analysis moreover we have also succeeded in single cell analysis. We believe that true personalised medicine is based of detailed characterisation of malignant cells of each patient with the potential to detect the most aggressive clones. From this prospective, single cell analysis gives the best opportunity to fulfil this need. The aim of our study is to compare transcriptomes of thousands CLL cells from consecutively collected samples of patients with aggressive disease with the aim to define the properties of subclones responsible for disease refractoriness.

Sustainable Development Goals

Masaryk University is committed to the UN Sustainable Development Goals, which aim to improve the conditions and quality of life on our planet by 2030.

Publications

Total number of publications: 20

2022

Gene expression analysis on single cell level uncovers the subclonal architecture of chromosomes in a leukemia patient with multiple chromosomal aberrations.

KOTAŠKOVÁ Jana KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina MAREČKOVÁ Andrea BOHÚNOVÁ Michaela PAVLOVÁ Šárka MALČÍKOVÁ Jitka NAVRKALOVÁ Veronika DOUBEK Michael PLEVOVÁ Karla POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Interrogating the molecular heterogeneity of chronic lymphocytic leukemia through computational approaches.

TAUŠ Petr KURUCOVÁ Terézia ZÁVACKÁ Kristýna PÁL Karol VALIŠOVÁ Martina KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka BRYJA Vítězslav JANOVSKÁ Pavlína PLEVOVÁ Karla

Conference abstract

Year: 2022, type: Conference abstract
Loss of heterozygosity in EIF3F gene leading to intellectual disability in two siblings.

VRZALOVÁ Zuzana HŮRKOVÁ Věra STAŇO KOZUBÍK Kateřina PÁL Karol TRKOVÁ Marie ŠTIKA Jiří BLAHÁKOVÁ Ivona RADOVÁ Lenka TRIZULJAK Jakub POSPÍŠILOVÁ Šárka DOUBEK Michael

Conference abstract

Year: 2022, type: Conference abstract
Přínos a úskalí sekvenačních metod v diagnostice hematologických malignit.

PLEVOVÁ Karla ZÁVACKÁ Kristýna PÁL Karol TAUŠ Petr STRÁNSKÁ Kamila REIGL Tomáš PORC Jakub Paweł HYNŠT Jakub PAVLOVÁ Šárka NAVRKALOVÁ Veronika ŽENATOVÁ Marcela KOTAŠKOVÁ Jana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Realita zapojení molekulárně biologických metod do diagnostiky a stratifikace lymfoproliferativních onemocnění.

KOTAŠKOVÁ Jana PLEVOVÁ Karla JAROŠOVÁ Marie POUR Luděk JANÍKOVÁ Andrea DOUBEK Michael POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Single-cell RNA sequencing analysis of T helper cell differentiation and heterogeneity

JAROUŠEK Radim MIKULOVÁ Antónia DAĎOVÁ Petra TAUŠ Petr KURUCOVÁ Terézia PLEVOVÁ Karla TICHÝ Boris KUBALA Lukáš

Article in Periodical

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, year: 2022, volume: 1869, edition: 10, DOI
Současná role cytogenomiky v diagnostice a prognostické stratifikaci hematologických malignit.

JAROŠOVÁ Marie KOTAŠKOVÁ Jana PLEVOVÁ Karla ONDROUŠKOVÁ Eva NAVRKALOVÁ Veronika BRYJOVÁ Lenka FIDRICHOVÁ Anna ČÁBELOVÁ Kateřina BOHÚNOVÁ Michaela ŠMEJKAL Jiří ŠMUHAŘOVÁ Petra OLTOVÁ Alexandra BORSKÝ Marek JEŽÍŠKOVÁ Ivana POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract
Subclonal architectureof chromosomes revealed by single-cell analysis of gene expression in a patient with clonal evolution of relapsing/refractory CLL

KOTAŠKOVÁ Jana KURUCOVÁ Terézia RÉBLOVÁ Kamila VALIŠOVÁ Martina ZÁVACKÁ Kristýna MAREČKOVÁ Andrea BOHÚNOVÁ Michaela PAVLOVÁ Šárka MALČÍKOVÁ Jitka NAVRKALOVÁ Veronika JAROŠOVÁ Marie DOUBEK Michael PLEVOVÁ Karla POSPÍŠILOVÁ Šárka

Conference abstract

Year: 2022, type: Conference abstract

2021

B cell developmental trajectory modeling – Using complex immunophenotype to identify pre-malignant CLL B cell subsets in peripheral blood of healthy donors.

JANOVSKÁ Pavlína STANCIKOVA J STUCHLÝ Jakub BAKARDJIEVA M. PLEVOVÁ Karla HRABČÁKOVÁ Viera VALIŠOVÁ Martina PLEŠINGEROVÁ Hana MIKULOVÁ Antónia KOTAŠKOVÁ Jana KALINA T. BRYJA Vítězslav

Conference abstract

Year: 2021, type: Conference abstract
Data mining of publicly available scRNA-seq datasets: application of machine learning to interrogate normal cellular counterparts of chronic lymphocytic leukemia.

TAUŠ Petr KURUCOVÁ Terézia VALIŠOVÁ Martina BRYCHTOVÁ Yvona PLEVOVÁ Karla KOTAŠKOVÁ Jana BRYJA Vítězslav JANOVSKÁ Pavlína

Conference abstract

Year: 2021, type: Conference abstract